注
需先运行‘数据准备’和‘差异表达分析’步骤，才可运行其他功能。如果不是从本公司进行分析的数据，客户需提供Reads数、FPKM值和TPM值的表达矩阵。如果仅提供Reads数的表达矩阵，则FPKM值和TPM值的相关功能无法使用。
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If using our platform in publication, please cite:
Geekgene Bioinformatics Service Portal/Platform (http://bioinfo.geekgene.com.cn/)Summary
参考文献：
Adrian Alexa and Jorg Rahnenfuhrer (2016). topGO: Enrichment Analysis for Gene Ontology. R package version 2.30.1.McCarthy, D. J., and G. K. Smyth. 2009. “Testing significance relative to a fold-change threshold is a TREAT.” Bioinformatics 25:765–71.
Wu, D., and G. K. Smyth. 2012. “Camera: a competitive gene set test accounting for inter-gene correlation.” Nucleic Acids Res 40 (17):e133.
Picelli S, Faridani O R, Björklund Å K, et al. Full-length RNA-seq from single cells using Smart-seq2[J]. Nature protocols, 2014, 9(1): 171.
Kapteyn J, He R, McDowell E T, et al. Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples[J]. BMC genomics, 2010, 11(1): 413.
Liu Y, Nie H, Liu H, et al. Poly (A) inclusive RNA isoform sequencing (PAIso− seq) reveals wide-spread non-adenosine residues within RNA poly (A) tails[J]. Nature communications, 2019, 10(1): 1-13.
Picelli S, Björklund Å K, Faridani O R, et al. Smart-seq2 for sensitive full-length transcriptome profiling in single cells[J]. Nature methods, 2013, 10(11): 1096.
Vieth, B., et al., A systematic evaluation of single cell RNA-seq analysis pipelines.Nat Commun, 2019. 10(1): p. 4667.
Love, M.I., Huber, W., Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Genome Biology 15(12):550 (2014)
T Wu, E Hu, S Xu, M Chen, P Guo, Z Dai, T Feng, L Zhou, W Tang, L Zhan, X Fu, S Liu, X Bo, and G Yu. clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. The Innovation. 2021, 2(3):100141
Gu, Z. (2016) Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics.
H. Wickham. ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag New York, 2016.
Durinck, S., P. Spellman, E. Birney, and W. Huber. 2009. “Mapping identifiers for the integration of genomic datasets with the R/Bioconductor package biomaRt.” Nature Protocols 4:1184–91.
Robinson, M. D., and A. Oshlack. 2010. “A Scaling Normalization Method for Differential Expression Analysis of RNA-seq data.” Genome Biology 11:R25.
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